Amino acid type-selective 15N/ 1H correlations for 13C, 15N labeled proteins

The pulse programs (VARIAN format) presented here are amino acid type-selective triple resonance experiments.

The pulse programs are discussed in detail in our: publications 


In order to find out how many signals are expected in each spectrum use the program SMASH which was developed at the FMP in Berlin by Ruediger Winter and Dirk Labudde.

For a description of every single experiment have a look at my PhD thesis or our publications.
You can either download my thesis as a pdf-file or if you live in Germany you can order a hardcopy from every bookshop (ISBN 3-89820-336-0, it is listed in "Verzeichnis Lieferbarer Bücher").

12 experiments are already running in the Protein-Pack format at NANUC  in Edmonton/Canada. I was at NANUC in February and wrote them with the help of Ryan McKay  . They are based on the "gcbca_co_nh" and "gcbca_nh". Several experiments can be recorded with one pulse sequence depending on which flags are set. However the 'TROSY' flag does not seem to work yet and we want to make sure that setting the decoupling power is save, before we distribute them. Feel free to contact Ryan McKay or M. Schubert

The following experiments are based on pulse sequences from Lewis Kay's lab and were implemented on a 500MHz maschine. (parameter files and shapes for 500MHz). The pulse sequences for the A(i+1)- and A(i,i+1)-HSQC are already in a final version. Crude versions of the other experiments are provided which means the experiments work fine, just the code contains still some unused parameters, comments are not updated etc.

Legal conditions

All pulse programs are freely available for everybody. However, if you successfully use them for the assignment of a protein and it results in a publication, you must cite our corresponding paper(s).

Pulse Programs

Experiment  pulse sequence  progpar-file shapes  selective for  reference 
G (i+1)   ma_glyco.c  G_i+1.par.tar  A_i+1_shapes.tar  Gly   JMR 1999  
G (i,i+1)   ma_glyca2.c  G_ii+1.par.tar  A_i+1_shapes.tar  Gly   JMR 1999  
A (i+1)   ma_A_i+1_c.c  A_i+1_HSQC.par.tar  A_i+1_shapes.tar  Ala   JMR 1999  
A (i,i+1)   ma_A_ii+1_k.c  A_ii+1_HSQC.par.tar  A_i+1_shapes.tar  Ala   JMR 1999  
TAVI (i+1)   ma_tavico.c  TAVI_i+1.par.tar  A_i+1_shapes.tar  Thr, Val, Ile and Ala   JMR 1999  
TAVI (i,i+1)   ma_tavica.c  TAVI_ii+1.par.tar  A_i+1_shapes.tar  Thr, Val, Ile and Ala   JMR 1999  
TA (i+1)   ma_taco.c  TA_i+1.par.tar  TA_i+1_shapes.tar  Thr and Ala   JMR 1999  
TA (i,i+1)   ma_taca.c  TA_ii+1.par.tar  TA_ii+1_shapes.tar  Thr and Ala   JMR 1999  

S (i+1)   ma_serco2.c  S_i+1.par.tar  S_i+1_shapes.tar  Ser   JMR 2001  
S (i,i+1)   ma_serca2.c  S_ii+1.par.tar  S_ii+1_shapes.tar  Ser   JMR 2001  
VIA (i+1)   ma_viaco.c  VIA_i+1.par.tar  VIA_i+1_shapes.tar  Val, Ile and Ala   JMR 2001  
VIA (i,i+1)   ma_viaca.c  VIA_ii+1.par.tar  VIA_ii+1_shapes.tar  Val, Ile and Ala   JMR 2001  
LA (i+1)   ma_laco4.c  LA_i+1.par.tar  LA_i+1_shapes.tar  Leu and Ala (weak Val, rarely Ile or Thr)   JMR 2001  
LA (i,i+1)   ma_laca2.c  LA_ii+1.par.tar  LA_ii+1_shapes.tar  Leu and Ala (weak Val, rarely Ile or Thr)  JMR 2001  

back to my homepage

Last modified March 27, 2003.