In order to find out how many signals are expected in each spectrum use the program
SMASH
which was developed at the FMP in Berlin by Ruediger Winter and
Dirk Labudde.
For a description of every single experiment have a
look at my PhD thesis or our publications.
You can either download
my thesis as a pdf-file or if you live in Germany you can order a hardcopy
from every bookshop (ISBN 3-89820-336-0, it is listed in "Verzeichnis Lieferbarer Bücher").
12 experiments are already running in the Protein-Pack format at
NANUC in Edmonton/Canada.
I was at NANUC in February and wrote them with the help of
Ryan McKay .
They are based on the
"gcbca_co_nh" and "gcbca_nh". Several experiments can be recorded with one pulse sequence
depending on which flags are set. However the 'TROSY' flag does not seem to work yet and we
want to make sure that setting the decoupling power is save, before we distribute them.
Feel free to contact Ryan McKay or
M. Schubert
The following experiments are based on pulse sequences from
Lewis Kay's lab
and were implemented on a 500MHz maschine. (parameter
files and shapes for 500MHz). The pulse sequences for the A(i+1)- and A(i,i+1)-HSQC
are already in a final version.
Crude versions of the other experiments are provided which means the experiments work fine,
just the code contains still some unused parameters, comments are not
updated etc.
Legal conditions
All pulse programs are freely available for everybody. However, if you successfully use them for
the assignment of a protein and it results in a publication, you must cite our corresponding paper(s).
Pulse Programs
| Experiment |
pulse sequence |
progpar-file |
shapes |
selective for |
reference |
| G (i+1) |
ma_glyco.c |
G_i+1.par.tar |
A_i+1_shapes.tar |
Gly |
JMR 1999 |
| G (i,i+1) |
ma_glyca2.c |
G_ii+1.par.tar |
A_i+1_shapes.tar |
Gly |
JMR 1999 |
| A (i+1) |
ma_A_i+1_c.c |
A_i+1_HSQC.par.tar |
A_i+1_shapes.tar |
Ala |
JMR 1999 |
| A (i,i+1) |
ma_A_ii+1_k.c |
A_ii+1_HSQC.par.tar |
A_i+1_shapes.tar |
Ala |
JMR 1999 |
| TAVI (i+1) |
ma_tavico.c |
TAVI_i+1.par.tar |
A_i+1_shapes.tar |
Thr, Val, Ile and Ala |
JMR 1999 |
| TAVI (i,i+1) |
ma_tavica.c |
TAVI_ii+1.par.tar |
A_i+1_shapes.tar |
Thr, Val, Ile and Ala |
JMR 1999 |
| TA (i+1) |
ma_taco.c |
TA_i+1.par.tar |
TA_i+1_shapes.tar |
Thr and Ala |
JMR 1999 |
| TA (i,i+1) |
ma_taca.c |
TA_ii+1.par.tar |
TA_ii+1_shapes.tar |
Thr and Ala |
JMR 1999 |
|
|
|
|
|
|
| S (i+1) |
ma_serco2.c |
S_i+1.par.tar |
S_i+1_shapes.tar |
Ser |
JMR 2001 |
| S (i,i+1) |
ma_serca2.c |
S_ii+1.par.tar |
S_ii+1_shapes.tar |
Ser |
JMR 2001 |
| VIA (i+1) |
ma_viaco.c |
VIA_i+1.par.tar |
VIA_i+1_shapes.tar |
Val, Ile and Ala |
JMR 2001 |
| VIA (i,i+1) |
ma_viaca.c |
VIA_ii+1.par.tar |
VIA_ii+1_shapes.tar |
Val, Ile and Ala |
JMR 2001 |
| LA (i+1) |
ma_laco4.c |
LA_i+1.par.tar |
LA_i+1_shapes.tar |
Leu and Ala (weak Val, rarely Ile or Thr) |
JMR 2001 |
| LA (i,i+1) |
ma_laca2.c |
LA_ii+1.par.tar |
LA_ii+1_shapes.tar |
Leu and Ala (weak Val, rarely Ile or Thr) |
JMR 2001 |